Prof.Dr. Roselyn Mary BILL

Aston University
School of Life & Health Sciences
Cellular and Molecular Biomedicine

Abstract
Membrane protein structural biology is critically dependent upon the supply of high-quality protein. Over the last few
years, the value of crystallising biochemically characterised, recombinant targets that incorporate stabilising mutations has
been established. Nonetheless, obtaining sufficient yields of many recombinant membrane proteins is still a major
challenge. Solutions are now emerging based on an improved understanding of recombinant host cells; as a ‘cell factory’ each cell is tasked with managing limited resources to simultaneously balance its own growth demands with those
imposed by an expression plasmid. My talk examines emerging insights into the role of translation and protein folding in
defining high-yielding recombinant membrane protein production in a range of host cells