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Workshop „Advanced Cell Culture Technologies”

ABOUT

What to expect Biomaterials

This workshop will include keynote lectures, short talks, poster presentations, round-table discussions and tech-demos on the topic of Advanced Cell Culture Technologies in the context of cell-based therapy products. We aim to bring together PhD students and Postdocs from this field to exchange knowledge, foster cooperation and discuss current and future needs and challenges in the field of culture technologies for cell-based therapy products. We will keep the workshop small and interactive – allowing a maximum of 60 participants.

Topics

We invite submissions for oral and poster presentations including but not limited to the following topics:

  • Scaffold-free and Scaffold-based Culture Systems
  • Dynamic bioreactor systems and monitoring
  • Imaging of 3D cell cultures
  • Media and supplements

The registration is now closed!

When

Friday, March 17, 2023

Where

University of Natural Resources and Life Sciences (BOKU), Muthgasse 11, Seminar Room 04, ground floor. (We recommend to travel via public transportation - U4 to Heiligenstadt. The venue is 10 minutes walking distance from the train station.)

Your organizers/hosts:

CorneliaUndEgger Cornelia Kasper,
Head, Institute of Cell & Tissue Culture Technologies (BOKU)
Head of ÖGMBT Working Group Cell Based Assays, Therapies & Products 		 ÖGMBT working groups Logo
Dominik Egger, Deputy Head, Institute of Cell & Tissue Culture Technologies (BOKU) Farhad Chariyev-Prinz, Institute of Cell & Tissue Culture Technologies (BOKU) Logo Institute name small
Supported by ÖGMBT (Austrian Association of Molecular Life Sciences and Biotechnology) logo

PROGRAM

Program

Last Update 14.3.2023: Time updates & Heuigen details added

09:00 - 10:30

Session 1: Keynote Lectures

Chair: Cornelia Kasper (BOKU, AT)

Antonina LavrentievaAntonina Lavrentieva (Leibniz Universität Hannover, DE)
Creation and characterization of 3D cell cultures of higher complexity: gradient hydrogels and hypoxia sensor cells

Jan HansmannJan Hansmann (Fraunhofer ISC & Technical University of Applied Sciences Würzburg-Schweinfurt, DE)
How engineering technologies support life science applications
10:30 - 10:40 Coffee Break
10:40 - 12:00

Session 2: Short talks

Chair: Dominik Egger (BOKU, AT)

S2-ST01 - Beate Rinner (Medical University of Graz, AT)

3D patient-derived tumor models to recapitulate the in vivo situation

S2-ST02 - Andrea De Luna (University for Continuing Education, AT)

Isolation and culture of Hoffa-derived adipose MSCs for extracellular vesicle production in a vertical wheel bioreactor

S2-ST03 - Sabrina Nebel (University of Natural Resources and Life Sciences BOKU, Vienna, AT)

3D cultivation of adipose-derived mesenchymal stem cells within alginate core–shell capsules

S2-ST04 - Oliver Kopinski-Grünwald (Technische Universität Wien- 3D Printing and Biofabrication, AT)

Self-assembling microscaffold-reinforced spheroids for bottom up orthopedic tissue repair

S2-ST05 - Janine Tomasch (University of Applied Sciences Technikum Wien, AT)

Comparison of the effects of cyclic and static tensile stress on skeletal muscle regeneration in a tissue-engineered model
12:00 Networking Lunch + Exhibition
13:15 Tech Demo
13:30 - 14:30

Round table discussions (5 topics, Current trends and novel methods)

Table 1: Automation & AI in culture technologies (Jan Hansmann)

What to expect: The automation of cell culture devices, such as bioreactors or incubators, and entire production chains unquestionably required to assure standardized cell therapy products. In this context, AI is becoming an increasingly valuable tool. At this table, we want to discuss where automation and AI can already assist on the basic research level and what the current limitations and future challenges of these technologies are.

Table 2: Primary cells of the future (Dominik Egger)

What to expect: Primary cells have a higher relevance compared to cell lines or genetically modified cells when it comes to therapeutic applications or the investigation of pathways or biological functions. However, the isolation and amplification (expansion) of these cells is comparatively complex and costly. At this table, we want to identify and discuss current challenges and needs in the field of primary cell isolation with a focus on what the workflows for the handling of primary cells could look like in the future.

Table 3: Hydrogels in 3D cell culture (Farhad Chariyev-Prinz)

What to expect: Employment of hydrogel systems is essential for the mimicry of an in vivo like environment. And although such 3D culture strategies have been shown to provide physiologically more relevant scientific outcomes, the broad employment of such platforms is yet to be established. At this roundtable we would like to discuss advantages and potential road blocks on the way to broad utilization of hydrogel-based 3D cultures and debate possible solutions to accelerate this trend.

Table 4: Dynamic culture systems and processes (Cornelia Kasper)

What to expect: Static cell culture format in standard well plates and petri dishes are still widely used in most cell culture labs. In context of the development of cell therapies and cell based products for advanced therapies it is of utmost importance to provide physiological relevant microenvironment reflecting physical, chemical and structural cues. At this table characteristics of different dynamic culture systems and manufacturing processes components will be discussed.

Table 5: Monitoring and Imaging in 3D cell culture (Antonina Lavrentieva)

What to expect: The analyses and assessment of 3D cell cultures is complex and often limited due to geometrical complexity, diffusion of compounds, light scattering and/or auto-fluorescence. Furthermore, many useful methods are invasive, altering the culture itself, or are endpoint measurements, not allowing to dynamically monitor the same sample over time. At this table we want to identify the current challenges and limitations and discuss existing and future approaches for the monitoring of 3D cell cultures.

14:30 - 14:45 Coffee Break
15:00 - 15:40

Session 3: Company Presentations

Chair: Farhad Chariyev-Prinz (BOKU, AT)

S3-ST01 - Philipp Paulitschke (Ludwig-Maximilians-Universität München, DE)

New non-invasive, label-free monitoring approach for 2D and 3D cell culture

S3-ST02 - Silke Isenhardt (PL BioScience GmbH, DE)

Moving towards animal-free cell culture media: Human platelet lysate, an innovative solution for FBS

S3-ST03 - Peter Frost (PELOBiotech GmbH - more than competence for cells, DE)

How Advanced 3D Cell Culture Systems are the solution for more Predictive and Reproducible Results
15:30 - 16:30

Poster Session

Please be at your poster during the poster session for Q&A.

PP-PP01 - Michaela Dehne (University of Augsburg / Institute of Physics, DE)

Establishment of a perfusion process with CHO cells using a 3D-printed spiral cell separator with automated flushing and online flow control

PP-PP02 - Jonathan Nyenhuis (Universität Augsburg, DE)

Development of an integrated optical Biosensor for Virus Detection in Cell Culture Processes

PP-PP03 - Marica Markovic (TU Wien, AT)

Extrusion-based bioprinting: a novel method for evaluating the volume of cell-laden 3D structures

PP-PP04 - Sabrina Summer (University for Continuing Education Krems, AT)

Mesenchymal stem cells support the formation of vascular sprouts by endothelial cells in human platelet lysate-based matrices

PP-PP05 - Julia Raitanen (LBI Applied Diagnostics, AT)

Comparison of radiation response between 2D and 3D cell culture models of different human cancer cell lines

PP-PP06 - Ilias Nikolits (University of Natural Resources and Life Sciences Vienna, AT)

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose For In Vitro Cultivation of Encapsulated Human MSCs

PP-PP07 - Katharina V. Meyer (Leibniz University Hannover, DE)

3D-printed microfluidic perfusion system for parallel monitoring of hydrogel-embedded cell cultures

PP-PP08 - Oscar Fabian Garcia Aponte (University of Natural Resources and Life Sciences, AT)

Development of a high throughput methylcellulose/gelatin methacrylate (gelma) fluidics-based encapsulation method for mesenchymal stem cells culturing

PP-PP09 - Ioana-Sonya Ciulean (Fraunhofer IZI, DE)

Development of a 3-dimensional head and neck cancer cell culture model for the assessment of NK cell-based therapies

PP-PP10 - Nicolas Debener (Leibniz University Hannover, DE)

Integration of fiber optical sensors into microsystems for the detection of specific biofilm patterns

PP-PP11 - Melina Hardt (Medical University of Graz, AT)

Establishing of 3D cell culture models to study infectious diseases and cancer

PP-PP12 - Julia Moldaschl (University of Natural Resources and Life Sciences (BOKU), AT)

3D multilineage differentiation model of mesenchymal stem/stromal cells under physiological conditions

PP-PP13 - Christina Windhaber (University of Veterinary Medicine Vienna, AT)

Three-dimensional equine colon enteroids – one step closer to mimicking the intact intestinal epithelium

PP-PP14 - Izaskun Itxaso (QPS Austria GmbH, AT)

Neuroinflammatory response of human differentiated microglia from healthy and Alzheimer disease patient
16:30 Poster Prize and closing
18:00

„Get together“ at Brandauers Bierbögen

Heiligenstädter Str. 31, 1190 Wien, directly located at U4/U6 station “Spittelau”.

 

REGISTRATION & ABSTRACTS

Registration & Abstract Submission

Registration Fees:

Fees in €uroÖGMBT MembersNon ÖGMBT members

Students*

30

60

Regular

50

130

(*) A confirmation of your enrolment as student at an institution of higher learning or a corresponding letter from your supervisor needs to be uploaded in PDF format during the registration process.

The registration fee includes:

  • Access to the workshop
  • Food & beverage at lunch and coffee break
  • Access to online abstract book
  • Access to online certificate of attendance

The registration is now closed!

Registration deadline (or before if all spaces are filled): March 6, 2023!

Payment:

For ÖGMBT members with an active and paid membership a member discount voucher code is available after login in the ÖGMBT intranet and can be entered during the payment process.

All payments are made via the online registration system (login box at the right of the top navigation menu) and can be made at the end of the registration process. Please note that payment of registration fees is required before abstract submission!

We offer several options for online payment which can be selected when placing an order (available options may depend on country) via our payment processor Mollie logo (Mollie B.V.) :

EPS EPS SOFORT SOFORT Giropay Giropay ApplePay Apple Pay*
MasterCard MasterCard AmericanExpress American Express VISA VISA Maestro Maestro
SEPA SEPA bank transfer ** (SEPA-countries only!)

* Apple Pay will only be available as an option on the payment screen if a supported device is used.

** SEPA bank transfers are only supported from SEPA Countries (mainly EU)!.

Cancellations

Due to the low registration fees, we cannot make refunds in case of cancellation, but you can name a substitute in your stead.

Call for Abstracts

Abstract submission is now closed! All submitted abstracts are currently being evaluated and submitters will soon be notified via e-mail about their abstract presentation status

Abstracts we are seeking: We invite submissions for oral and poster presentations including but not limited to the following topics:

  • Scaffold-free and Scaffold-based Culture Systems
  • Dynamic bioreactor systems and monitoring
  • Imaging of 3D cell cultures
  • Media and supplements

Abstract submission: After completion of your registration you can login to the online registration system (login box at top right of the top navigation menu) and submit an optional abstract. All abstracts must be submitted using the online registration system and require payment of the registration fee before submission.
Please prepare an abstract consisting of a title (250 characters max.), up to 14 authors and an abstract body (2500 characters max.). In the online editor you may use simple formatting elements such as bold, italic, underline, super & subscript and special characters in both the title and abstract. No images or tables are allowed.

Poster prize: all posters presented will compete for a poster prize (one free registration for the 15th ÖGMBT Annual Meeting, Salzburg, 19-21.9.2023)

Antonina Lavrentieva (Leibniz Universität Hannover, DE)

Antonina Lavrentieva

Antonina Lavrentieva is a group leader at the Institute of Technical Chemistry, Leibniz University of Hannover, working in the field of stem cell research, biotesting and tissue engineering. In 2022 she received the venia legendi in Technical Chemistry. In her second PhD Thesis, she studied methods of expanding mesenchymal stem cells (MSCs) in bioreactors, as well as the influence of hypoxia on the MSCs. She studied Biology and Life Science at Moscow State University and the Leibniz University of Hannover. She also defended a PhD Thesis in Physiology. Her current research interests include stem cell media optimization, 3D cell culture, implementation of genetically encoded sensors for 3D cell culture characterization, gradient hydrogels for studying stem cell niches and 3D-bioprinting. Currently, she is the head of advisory board of DECHEMA professional group "Medical biotechnology".

Jan Hansmann (University of Applied Sciences Würzburg-Schweinfurt, DE)

Jan Hansmann

Jan Hansmann studied Technical Cybernetics at the University Stuttgart. He conducted his Ph.D. dissertation at the Fraunhofer IGB. Following graduation, Jan Hansmann established his own group focused on developing technology platforms to facilitate generation and characterization of tissues in vitro. In 2013, Hansmann acquired funding from the German Federal Ministry of Education and Research (BMBF) to start his own junior research group at the University Hospital Würzburg. In addition, he was heading a group for Automation in Life Sciences at the Fraunhofer ISC, Würzburg. Since 2018, Jan Hansmann is a professor at the Technical University for Applied Sciences Würzburg/Schweinfurt. His activities at the Fraunhofer ISC are still ongoing. His scientific activities combine engineering, materials science, medicine, and biology.

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